Worthington

Collagenase

Clostridium histolyticum contains two distinct but related genes for collagenase. The col G gene codes for a 936-amino acid protein designated Collagenase Type 1 and the col H gene codes for a 1021 amino acid protein designated Collagenase Type II. As a result of the correlations, several types of partially purified collagenases have been established by Worthington: Types 1, 2, 3, 4, 5, 6 and 7.

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Deoxyribonuclease I

Worthington prepares Deoxyribonuclease I from bovine pancreas at different levels of purity to suit many different applications.

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Papain

Worthington Papain is purified from Carica papaya latex. This enzyme has wide specificity, and it will degrade most protein substrates more extensively than the pancreatic proteases.

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Trypsin

Worthington purifies trypsin from bovine pancreas. Our enzyme is extensively used in protein sequencing work and for tissue dissociation.

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Elastase

Worthington Elastase is prepared from porcine pancreas. It is extensively used in tissue and cell dissociation procedures.

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Ribonuclease

Worthington Ribonucleases A & B are prepared from bovine pancreas and are offered in several grades to suit various applications. When Ribonuclease A is used to remove RNA during DNA isolation, the enzyme must be free of deoxyribonuclease activity to prevent damage to the DNA.

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BioVectra

IPTG

IPTG is a molecular biology reagent. This compound is a molecular mimic of allolactose, a lactosemetabolite that triggers transcription of the lac operon, and it is therefore used to induce protein expression where the gene is under the control of the lac operator.

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X-Gal

X-gal is often used in molecular biology to test for the presence of an enzyme, β-galactosidase. It is also used to detect activity of this enzyme in histochemistry and bacteriology.

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X-Gluc

X-Gluc is used as a reagent to detect β-glucuronidase, an enzyme produced by the E. coli bacterium.

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